Pueraria mirifica , also known as Kwao Krua (but not exclusively), is a plant found in northern and north eastern Thailand and Myanmar.In Thailand, the plant known as “Kwao Krua” is considered to be a rejuvenating adaptogenic herb and has a history of use in folk medicine. Although the name “Kwao Krua” had been applied to several species of plants having tuberous roots, it was definitively identified as Pueraria mirifica in 1952. An unusual estrogenic phenol, miroestrol, was isolated eight years later from this plant.

Womens' health, Oestrogen supplement, breast enlargement, skin firming and nourishment, blood circulation, energy and vigor

Benefits:

• Provides healthier, stronger hair and nails
• Decreases menopausal hot flushes and sweating
• Increase energy and vigor, more reflexive bodily movements
• Increase blood circulation
• Alleviate sleep disorders
• Breast Enlargement
• Anti-aging & Ant-Wrinkle Properties

Pueraria mirifica Airy Shaw & Suvatabandhu is a climbingplant known in Thailand as White Kwao Krua. Theancestral uses of its tuberous root in folklore medicine forpromoting vigor and rejuvenating have been mentioned inThai traditional text books. It has been established thatthe rejuvenating effect of P. mirifica was mediated by anestrogenic action of miroestrol (Pope et al., 1958; Cain,1960) and several active isoflavones such as daidzin,daidzein, genistin, genistein and puerarin (Chansakaowet al., 2000). In recent years P. mirifica is gainingpopularity in Thailand as researches continue to pour insupporting its potent estrogenic effect and non-toxicity(Cherdshewasart, 2003; Cherdshewasart et al., 2004;Malaivijitnond et al., 2004; Trisomboon et al., 2004). Withrespect to its beneficial properties based on its richlyphytoestrogenic content, P. mirifica has been popularlyused as a major ingredient in variety of cosmeticproducts, for instance, breast cream, eye gel, skin moisturizer and hair tonic. Recently, it has been proposedas a new alternative to the use of postmenopausalhormone replacement therapy (Muangman andCherdshewasart, 2001).

Although a number ofresearches have been done in regards to estrogenicactivity of P. mirifica, the variation of isoflavonoids inKwao Kruas at different ages and different harvestedlocations has been reported (Manosroi and Manosroi,2003). This study, thus, carried out to investigate theestrogenic activity of P. mirifica powder purchased fromLampang Province, one of the areas that this plant isharvested for using as raw material in herbal industries.The estrogenic activity test was conducted by bioassay inovariectomized mice in comparison to genuineestrogen.

Reference
- Pope GS, Grundy HM, Jone HEH, Tait SAS (1958). The oestrogenicsubstance (miroestrol) from the tuberous roots of P. mirifica. J.Endocrinol. 1958 (17): 15-16.
- Cain JC (1960). Miroestrol: An oestrogen from the plant Puerariamirifica, Nat. 188: 774-777.
- Chansakaow S, Ishikawa T, Sekine K, Okada M, Higuchi Y, Kudo M,Chaichantipyuth C (2000). Isoflavonoids from Pueraria mirifica andtheir estrogenic activity. Planta Med. 66: 572-575.

• Serves as ''fountain of youth''
• Darkens grey hair and helps increasing hair growth
• Increases energy levels and vigor
• Better blood circulation
• Diminues sleep problems
• It will help your skin to look smoother and younger

Estrogen bioassy of Pueraria mirifica Airy Shaw & Suvatabandhu
Tanapan Siangcham, Supap Saenphet and Kanokporn Saenphet*
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.

Abstract

Pueraria mirifica Airy Shaw & Suvatabandhu has been used by Thai women for centuries as arejuvenating herb. The scientific claim for its phytoestrogenic property leads to the popular use of thisplant in several herbal formulations. This research project was carried out to examine estrogenicactivity of P. mirifica from Lampang Province, Thailand, one of the areas that it is harvested for using asraw material in herbal industries. Estrogen bioassay of aqueous extract from P. mirifica atconcentrations of 0.1 and 0.2 g/ml was performed in immature ovariectomized mice in comparison toboth the negative and positive control groups. Vaginal orifice of mice treated with the extract in allgroups and with the positive control group which injected with estradiol benzoate (EB) opened earlierthan the negative control mice and vaginal cornification was also found in these 3 groups only.Moreover, the extract significantly increased uterine weight and uterine epithelium height as comparedto negative control group with the comparable degree of increment to those of EB treated group. Thedata of this present study indicated that dried powder of P. mirifica which is used as raw material forherbal products of Lampang Province, exhibited the potent estrogenic activity.

Result
The effects of aqueous extract from dried powder of P.mirifica on the timing of vaginal opening and vaginalcytology in ovariectomized mice are shown in Table 1.Vaginal opening of mice treated with the extract at bothconcentrations used and with EB occurred in day 4 oftreatment, while control mice showed no signs of vaginalopening until day 7 of the experiment. Vaginal cytologyrevealed the stage of estrous in mice treated with theextracts and EB, showing abundant cornified cells. Incontrast, vaginal cell cornification was not induced incontrol mice. Administration of aqueous extract from P.mirifica could induce a significant increase in uterineweight . The uterotrophic effect of P. mirificawas also evident from the increase in the height ofluminal epithelium and the proliferation of glandularepithelium (Table 2 and Figure 1). The group of 0.2 g/mlshowed a tentative of higher uterotrophic activity than EBgroup.

Major isoflavonoid contents of the phytoestrogen rich-herb Pueraria mirifica in comparison with Pueraria lobata
Wichai Cherdshewasart , Subongkoj Subtang , Winai Dahlan
Department of Biology, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok 10330, Thailand
Bureau of Community Technology, Department of Science Service, Ministry of Science and Technology, Rama-VI Road, Phyathai, Bangkok 10400, Thailand
Faculty of Allied Health Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok 10330, Thailand

Abstract

Pueraria mirifica tubers collected from 28 out of 76 provinces of Thailand and Pueraria lobata tubers collected from Guangzhou province, China were submitted to HPLC analysis with the established gradient system comprising 1.5% acetic acid and acetonitrile. Five major isoflavonoids, including puerarin, daidzin, genistin, daidzein and genistein, were adopted as authentic standards. P. mirifica tubers showed intra- as well as inter-provincial differences in isoflavonoid and total isoflavonoid contents. The difference in both cases should be mostly influenced by genetic and environmental factors. In comparison with P. lobata, P. mirifica population exhibited differences only with a lower amount of daidzein. © 2006 Elsevier B.V. All rights reserved. Keywords: Pueraria mirifica; Pueraria lobata; Isoflavonoid; HPLC analysis; Chemovariety

Discussion
" The difference in levels of individual and total isoflavonoidcontents among tubers collected from different provinces inThailand or within the same province but in different districtsexhibited significant differences in the plants’ active chemicals.Such differences may be influenced more by genotype than theplant age or environmental/habitat factors. The variability inisoflavonoid levels in mature tubers could seriously affect thequality of any tuber-derived materials or even research resultsderived from the plants. The established information of theisoflavonoid contents could therefore provide a helpful guidelinefor plant breeders acting as benchmark for selection forhigh isoflavonoid contents followed by clonal propagation forcommercial plantations. It is possible to select for clones withhigher isoflavonoid contents than P. lobata, resulting in developmentof commercial products with greater estrogenic activities.Furthermore, there is strong interest in setting up a comparativeestrogenic activity study among the different plant samples tohelp select those with high estrogenic effects for the developmentof cosmetics, dietary supplements or pharmaceutical products.Furthermore, correlation analysis between isoflavonoid contentsand bio-activity of the plant extracts could help characterize achemical marker to identify plants with high estrogenic effects.The most interesting conclusion from this study is the geneticdifferences of the plants themselves. Chemovariety may alsoexist in the plant population. Molecular analysis at the level ofRAPD could help finding the phylogenic relationship at the varietylevel. The analysis of some selected genes in the isoflavonoidpathways could enhance our understanding of the synthesis andstorage of isoflavonoids in the plants and enable us to manipulatethe whole plant or plant tissue to produce a higher amountof isoflavonoids with more estrogenic effects. "

Estrogenic Effects of Pueraria mirifica on the Menstrual Cycle and Hormone-Related Ovarian Functions in Cyclic Female Cynomolgus Monkeys
Hataitip Trisomboon, Suchinda Malaivijitnond, Gen Watanabe and Kazuyoshi Taya
Biological Science Ph.D. Program, Faculty of Science, Chulalongkorn University
BPrimate Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology
Department of Basic Veterinary Science, The United Graduated School of Veterinary Science, Gifu University

Abstract

This study investigated the estrogenic effect of Pueraria mirifica (P. mirifica) on menstrual cycle length and hormone-related ovarian function. Nine normal cyclic monkeys (Macaca fascicularis) were separated into 3 groups; each group was force fed with a single dose of 10, 100, and 1,000 mg of P. mirifica. The experimental schedule was separated into the pre-treatment and post-treatment periods. Blood samples were collected on days 3, 9 – 14, 19, 24, 29, and every 10 days until the next menstruation for one and two menstrual cycles during two consecutive periods and assayed for serum levels of gonadotropins and ovarian hormones. The result showed a significant increase in lengths of the follicular phase and total menstrual cycle in monkeys treated with 1,000 mg of P. mirifica, but no change in menstrual cycle length in monkeys treated with 10 and 100 mg of P. mirifica. Serum levels of follicle stimulating hormone, luteinizing hormone, estradiol, progesterone, or immunoreactive-inhibin did not change during the first and second menstrual cycles of the post-treatment period for all monkey groups. Our findings demonstrate that although changes in hormonal levels could not be observed in this study, a single dose of 1,000 mg of P. mirifica can disturb ovarian function and menstrual cycle in monkeys. Keywords: Pueraria mirifica, phytoestrogen, monkey, reproductive hormone, menstrual cycle

Payment and Shipping

• We accept PAYPAL only.
• I will ship worldwide by Registered Airmail from Thailand .
• Express Mail (EMS) is optional. Extra cost will be applied on Shipping.
• Shipping time is normally around 7 - 21 days. On occasion, it might take more than 21 days depending on each country custom. Your patience is greatly appreciated.
• We will ship all items and post feedback within 3 days of payment.** IF You have any questions please Connect me via eBay message. I may try to resolve any problem you may have. Please Email me before leaving a Feed Back.

We accept PayPal payments only.

We gladly accept returns under the following conditions:

• Merchandise must be returned unopened within 14 days of receipt, in its original condition with the original packaging and packing slip.
• After we receive your item, you will receive a full refund less shipping and any gift-wrapping charges. Any shipping cost you incur to return the product will not be refunded.
• We do not offer refunds or exchanges on skin care, hair care, or make-up items due to health reasons.